marscan

 

Function

Finds MAR/SAR sites in nucleic sequences

Description

Matrix/scaffold attachment regions (MARs/SARs) are genomic elements thought to delineate the structural and functional organisation of the eukaryotic genome. Originally, MARs and SARs were identified through their ability to bind to the nuclear matrix or scaffold. Binding cannot be assigned to a unique sequence element, but is dispersed over a region of several hundred base pairs. These elements are found flanking a gene or a small cluster of genes and are located often in the vicinity of cis-regulatory sequences. This has led to the suggestion that they contribute to higher order regulation of transcription by defining boundaries of independently controlled chromatin domains. There is indirect evidence to support this notion. In transgenic experiments MARs/SARs dampen position effects by shielding the transgene from the effects of the chromatin structure at the site of integration. Furthermore, they may act as boundary elements for enhancers, restricting their long range effect to only the promoters that are located in the same chromatin domain.

marscan finds a bipartite sequence element that is unique for a large group of eukaryotic MARs/SARs. This MAR/SAR recognition signature (MRS) comprises two individual sequence elements that are <200 bp apart and may be aligned on positioned nucleosomes in MARs. The MRS can be used to correctly predict the position of MARs/SARs in plants and animals, based on genomic DNA sequence information alone. Experimental evidence from the analysis of >300 kb of sequence data from several eukaryotic organisms show that wherever a MRS is observed in the DNA sequence, the corresponding genomic fragment is a biochemically identifiable SAR.

The MRS is a bipartite sequence element that consists of two individual sequences of 8 (AATAAYAA) and 16 bp (AWWRTAANNWWGNNNC) within a 200 bp distance from each other. One mismatch is allowed in the 16 bp pattern. The patterns can occur on either strand of the DNA with respect to each other. The 8 bp and the 16 bp sites can overlap.

Where there are many possible MRS sites caused by many 8 bp and/or 16 bp pattern sites located within 200 bp of each other, then only the 8 bp site and the 16 bp site that occur closest to each other are reported.

Once a MRS has been reported, no more sites will be looked for within 200 bp of that site. This reduces (but maybe will not totally eliminate) over-reporting of the clusters of MRS's that tend to occur within a MAR/SAR.

Not all SARs contain a MRS. Analysis of >300 kb of genomic sequence from a variety of eukaryotic organisms shows that the MRS faithfully predicts 80% of MARs and SARs, suggesting that at least one other type of MAR/SAR may exist which does not contain a MRS.

It it still not at all clear whether MAR/SARs are real biological phenomena or just experimental artefacts.

The problem of how to define and find MARs is still being actively invetsigated. For a recent evaluation of this method and others, see reference 3.

Usage

Here is a sample session with marscan 


% marscan 
Finds MAR/SAR sites in nucleic sequences
Input sequence(s): tembl:u01317
Output report [hshbb.marscan]:

Go to the input files for this example
Go to the output files for this example

Command line arguments 

   Standard (Mandatory) qualifiers:
  [-sequence]          seqall     Sequence database USA
  [-outfile]           report     File for output of MAR/SAR recognition
                                  signature (MRS) regions. This contains
                                  details of the MRS in normal GFF format. The
                                  MRS consists of two recognition sites, one
                                  of 8 bp and one of 16 bp on either sense
                                  strand of the genomic DNA, within 200 bp of
                                  each other.

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-sequence" associated qualifiers
   -sbegin1             integer    Start of each sequence to be used
   -send1               integer    End of each sequence to be used
   -sreverse1           boolean    Reverse (if DNA)
   -sask1               boolean    Ask for begin/end/reverse
   -snucleotide1        boolean    Sequence is nucleotide
   -sprotein1           boolean    Sequence is protein
   -slower1             boolean    Make lower case
   -supper1             boolean    Make upper case
   -sformat1            string     Input sequence format
   -sdbname1            string     Database name
   -sid1                string     Entryname
   -ufo1                string     UFO features
   -fformat1            string     Features format
   -fopenfile1          string     Features file name

   "-outfile" associated qualifiers
   -rformat2            string     Report format
   -rname2              string     Base file name
   -rextension2         string     File name extension
   -rdirectory2         string     Output directory
   -raccshow2           boolean    Show accession number in the report
   -rdesshow2           boolean    Show description in the report
   -rscoreshow2         boolean    Show the score in the report
   -rusashow2           boolean    Show the full USA in the report

   General qualifiers:
   -auto                boolean    Turn off prompts
   -stdout              boolean    Write standard output
   -filter              boolean    Read standard input, write standard output
   -options             boolean    Prompt for standard and additional values
   -debug               boolean    Write debug output to program.dbg
   -verbose             boolean    Report some/full command line options
   -help                boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning             boolean    Report warnings
   -error               boolean    Report errors
   -fatal               boolean    Report fatal errors
   -die                 boolean    Report deaths

Standard (Mandatory) qualifiers Allowed values Default
[-sequence]
(Parameter 1)		 Sequence database USA Readable sequence(s) Required
[-outfile]
(Parameter 2)		 File for output of MAR/SAR recognition signature (MRS) regions. This contains details of the MRS in normal GFF format. The MRS consists of two recognition sites, one of 8 bp and one of 16 bp on either sense strand of the genomic DNA, within 200 bp of each other. Report output file  
Additional (Optional) qualifiers Allowed values Default
(none)
Advanced (Unprompted) qualifiers Allowed values Default
(none)

Input file format

marscan reads a normal genomic DNA USA.

Input files for usage example

'tembl:u01317' is a sequence entry in the example nucleic acid database 'tembl'

Database entry: tembl:u01317

ID   HSHBB      standard; DNA; HUM; 73308 BP.
XX
AC   U01317; J00093; J00094; J00096; J00158; J00159; J00160; J00161; J00162;
AC   J00163; J00164; J00165; J00166; J00167; J00168; J00169; J00170; J00171;
AC   J00172; J00173; J00174; J00175; J00177; J00178; J00179; K01239; K01890;
AC   K02544; M18047; M19067; M24868; M24886;
XX
SV   U01317.1
XX
DT   19-MAR-1994 (Rel. 39, Created)
DT   31-MAR-2001 (Rel. 67, Last updated, Version 28)
XX
DE   Human beta globin region on chromosome 11.
XX
KW   allelic variation; alternate cap site; Alu repeat; beta-1 pseudogene;
KW   beta-globin; delta-globin; epsilon-globin; gamma-globin; gene duplication;
KW   globin; HPFH; KpnI repetitive sequence; polymorphism; promoter mutation;
KW   pseudogene; repetitive sequence; RNA polymerase III; thalassemia.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo.
XX
RN   [1]
RP   62409-62631, 63482-63610
RX   MEDLINE; 74275150.
RA   Marotta C.A., Forget B.G., Weissman S.M., Verma I.M., McCaffrey R.P.,
RA   Baltimore D.;
RT   "Nucleotide sequences of human globin messenger RNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:2300-2304(1974).
XX
RN   [2]
RP   63602-63646
RX   MEDLINE; 76053173.
RA   Forget B.G., Marotta C.A., Weissman S.M., Cohen-Solal M.M.;
RT   "Nucleotide sequences of the 3'-terminal untranslated region of messenger
RT   RNA for human beta globin chain";
RL   Proc. Natl. Acad. Sci. U.S.A. 72:3614-3618(1975).
XX
RN   [3]
RP   63593-63626
RX   MEDLINE; 77022845.
RA   Proudfoot N.J., Brownlee G.G.;
RT   "Nucleotide sequences of globin messenger RNA";
RL   Br. Med. Bull. 32:251-256(1976).
XX
RN   [4]
RP   63673-63743
RX   MEDLINE; 77114153.
RA   Proudfoot N.J., Longley J.I.;


  [Part of this file has been deleted for brevity]

     aaaggggaga agaatcaaat agacgcaata aaaaatgaca cggggtatca ccactgatcc     70380
     cacagaaata caaactaccg tcagagaata ctataaacac ctctacgcaa ataaactaga     70440
     aaatctagaa gaaatggata aattcctcga cacatacact ctgccaagac taaaccagga     70500
     agaagttgta tctctgaata gaccaataac aggctctgaa attgaggcaa taattaatag     70560
     cttatcaacc aaaaaaagtc cgggaccagt aggattcata gccgaattct accagaggta     70620
     caaggaggag ctggtaccat tccttctgaa actattccaa tcaatagaaa aagagggaat     70680
     cctccctaac tcattttatg aggccagcat catcctgata ccaaagcctg acagagacac     70740
     aacaaaaaaa gagaatgtta caccaatatc cttgatgaac atcgatgcaa aaatcctcaa     70800
     taaaatactg gcaaactgaa tccagcagca catcaaaaag cttatcctcc atgatcaagt     70860
     gggcttcatc cctgccatgc aaggctggtt caacatacga aatcaataaa cataatccag     70920
     catataaaca gaaccaaaga cacaaaccat atgattatct caatagatgc agaaaaggcc     70980
     tttgacaaaa ttcaacaatg cttcatgcta aaaactctca ataaattagg tattgatggg     71040
     acatatctca aaataataag agctatctat gacaaaccca cagccaatat catactgagt     71100
     ggacaaaaac tggaagcatt ccctttgaaa actggcacaa ggcagggatg ccctctctca     71160
     ccactcctat tcaacatagt gttggaagtt ctggccaggg caatcaggca ggagaaggaa     71220
     ataaagggca ttcaattagg aaaagaggaa ggtgaaattg tccctgtttg cagatgacat     71280
     gattgtatat ctagaaaacc ccattgtctc agcccaaaat ctccttaagc tgataagcaa     71340
     cttcagcaaa gtctcaggat ataaaatcag tgtgcaaaaa tcacaagtat tcctatgcac     71400
     caataacaga caaacagaga gccaaatcat gagtgaactc ccattcacaa ttgcttcaaa     71460
     gagaataaaa tacctaggaa tccaacttac aagggatgtg aaggacctct tcaaggagaa     71520
     ctacaaacca ctgctcaatg aaataaaaga ggatacaaac aaatggaaga acattccatg     71580
     cttatgggta ggaagaatca tatcgtgaaa atggtcatac tgcccaaggt aatttataga     71640
     ttcaatgcca tccccatcaa gctaccaatg actttcttca cagaactgga aaaaactact     71700
     ttaaagttca tatggaatca aaaaagagcc cacatcacca aggcaatcct aagccaaaag     71760
     aacaaagctg gaggcatcac gctacctgac ttcaaactat actacaatgc tacggtaacc     71820
     aaaacagcat ggtactggta ccaaaacaga gatctagacc aatggaacag aacagagccc     71880
     tcagaaataa tgccgcatat ctacaactat ccgatctttg acaaacctga gagaaacaag     71940
     caatggggaa aggattccct atttaataaa tggtgctggg aaaactggct agccatatgt     72000
     agaaagctga aactggatcc ttccttacac cttatacaaa aattaattca agatggatta     72060
     aagacttaaa cattagacct aaaaccataa aaaccctaga aaaaaaccta ggcaatacca     72120
     ttcaggacat aggcatgggc aaggacttca tgtctaaaac accaaaacga atggcaacaa     72180
     aagacaaaat ggacaaacgg gatctaatta aactaaagag cttctgcaca gctaaagaaa     72240
     ctaccatcag agtgaacagg caacctacaa aatgggagaa aatttttgca atctactcat     72300
     ctgacaaagg gctaatatcc agaatctaca atgaactcaa acaaatttac aagaaaaaac     72360
     aaacaacccc atcaaaaagt gggcaaagga tatgaacaga cacttctcaa aagaagacat     72420
     ttatgtaatc aaaaaacaca tgaaaaaatg ctcatcatca ctagccatca gagaaatgca     72480
     aatcaaaacc acaatgagat accatctcac accagttaga atggcgatca ttaaaaagtc     72540
     aggaaacaac aggtgctgga gaggatgtgg agaaacagga acaactttta cactgttggt     72600
     gggactgtaa actagttcaa ccattgcgga agtcagtgtg gcaattcctc aggaatctag     72660
     aactagaaat accatttgac ccagccatcc cattactggg tagataccca aaggattata     72720
     aatcatgctg ctataaagac acatgcacac gtatgtttat tgcagcacta ttcacaatag     72780
     caaagacttg gaaccaaccc aaatgtccaa caacgataga ttggattaag aaaatgtggc     72840
     acatatacac catggaatac tatgcagcca taaaaaatga tgagttcatg tcctttgtag     72900
     ggacatggat gaagctggaa actatcattc tcagcaaact atcacaagga caataaacca     72960
     aacaccgcat gttctcactc ataggtggga attgaacaat gagaacacat ggacacatga     73020
     agaggaacat cacactctgg ggactgttat ggggtggggg gcaggggcag ggatagcact     73080
     aggagatata cctaatgcta aatgacgagt taatgggtgc agcacaccaa catggcacat     73140
     gtatacatat ataacaaacc tgccgttgtg cacatgtacc ctaaaacttg aagtataata     73200
     ataaaaaaaa gttatcctat taaaactgat ctcacacatc cgtagagcca ttatcaagtc     73260
     tttctctttg aaacagacag aaatttagtg ttttctcagt cagttaac                  73308
//

Output file format

The output is a standard EMBOSS report file.

The results can be output in one of several styles by using the command-line qualifier -rformat xxx, where 'xxx' is replaced by the name of the required format. The available format names are: embl, genbank, gff, pir, swiss, trace, listfile, dbmotif, diffseq, excel, feattable, motif, regions, seqtable, simple, srs, table, tagseq

See: http://emboss.sf.net/docs/themes/ReportFormats.html for further information on report formats.

By default marscan writes a GFF (Gene Feature Format) report file.

Output files for usage example

File: hshbb.marscan 

##gff-version 2.0
##date 2005-07-15
##Type DNA HSHBB
HSHBB	marscan	misc_signal	2242	2458	0.000	+	.	Sequence "HSHBB.1" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 2451" ; note "*end8bp 2458" ; note "*start16bp 2242" ; note "*end16bp 2257"
HSHBB	marscan	misc_signal	17654	17730	0.000	+	.	Sequence "HSHBB.2" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 17723" ; note "*end8bp 17730" ; note "*start16bp 17654" ; note "*end16bp 17669"
HSHBB	marscan	misc_signal	40956	41123	0.000	+	.	Sequence "HSHBB.3" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 40956" ; note "*end8bp 40963" ; note "*start16bp 41108" ; note "*end16bp 41123"
HSHBB	marscan	misc_signal	42232	42248	0.000	+	.	Sequence "HSHBB.4" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 42232" ; note "*end8bp 42239" ; note "*start16bp 42233" ; note "*end16bp 42248"
HSHBB	marscan	misc_signal	47834	47966	0.000	+	.	Sequence "HSHBB.5" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 47959" ; note "*end8bp 47966" ; note "*start16bp 47834" ; note "*end16bp 47849"
HSHBB	marscan	misc_signal	65112	65146	0.000	+	.	Sequence "HSHBB.6" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 65139" ; note "*end8bp 65146" ; note "*start16bp 65112" ; note "*end16bp 65127"
HSHBB	marscan	misc_signal	65947	65963	0.000	+	.	Sequence "HSHBB.7" ; note "*type MAR/SAR recognition site (MRS)" ; note "*start8bp 65947" ; note "*end8bp 65954" ; note "*start16bp 65948" ; note "*end16bp 65963"

Data files

None.

Notes

It does not check whether the DNA input sequence is genomic or not.

References

  1. The method for finding the MAR/SAR sites is described in:

    van Drunen CM., Sewalt RGAB., Oosterling RW., Weisbeek PJ., Smeekens SCM. and van Driel R. "A bipartite sequence element associated with matrix/scaffold attachment regions" Nucleic Acids Research. 1999. Vol 27, No. 14, pp. 2924-2930

  2. The original paper on MARs/SARs is:

    Mirkovitch J., Mirault M-E. and Laemmli UK. Cell. 1984. Vol. 39 pp. 223-232.

  3. A recent evaluation of methods to find MARs/SARs:

    I. Liebich, J. Bode, I. Reuter and E. Wingender "Evaluation of sequence motifs found in scaffold/matrix-attached regions (S/MARs)" Nucleic Acids Research 2002, Vol. 30, No. 15 3433-3442

Warnings

None

Diagnostic Error Messages

None.

Exit status

It always exits with status 0.

Known bugs

None.

See also

Program nameDescription
dregRegular expression search of a nucleotide sequence
fuzznucNucleic acid pattern search
fuzztranProtein pattern search after translation
getorfFinds and extracts open reading frames (ORFs)
plotorfPlot potential open reading frames
showorfPretty output of DNA translations
sixpackDisplay a DNA sequence with 6-frame translation and ORFs
sycoSynonymous codon usage Gribskov statistic plot
tcodeFickett TESTCODE statistic to identify protein-coding DNA
wobbleWobble base plot

Author(s)

Gary Williams (gwilliam © rfcgr.mrc.ac.uk)
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

History

Written (Jan 2001) - Gary Williams.

Changed output file to standard EMBOSS report format (April 2002) - Peter Rice

Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

Comments

None