EMBOSS explorer

needle

Needleman-Wunsch global alignment. (read the manual)

Input section

Select an input sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:

Select an input sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:

Matrix file. Use one of the following two fields:

  1. To access a standard EMBOSS data file, enter the name here: (default is EBLOSUM62 for protein, EDNAFULL for nucleic)
  2. To upload a data file from your local computer, select it here:
Required section
Gap opening penalty (default is 10.0 for any sequence)
Gap extension penalty (default is 0.5 for any sequence)
Output section
Brief identity and similarity?
Output alignment format
Run section
Email address: If you are submitting a long job and would like to be informed by email when it finishes, enter your email address here.